Blar i forfatter "Godtliebsen, Gustav"

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    • Analyzing Mitochondrial Morphology Through Simulation Supervised Learning 

      Punnakkal, Abhinanda Ranjit; Godtliebsen, Gustav; Somani, Ayush; Acuna Maldonado, Sebastian Andres; Birgisdottir, Åsa birna; Prasad, Dilip K.; Horsch, Alexander; Agarwal, Krishna (Journal article; Tidsskriftartikkel; Peer reviewed, 2023-03-03)
      The quantitative analysis of subcellular organelles such as mitochondria in cell fluorescence microscopy images is a demanding task because of the inherent challenges in the segmentation of these small and morphologically diverse structures. In this article, we demonstrate the use of a machine learning-aided segmentation and analysis pipeline for the quantification of mitochondrial morphology in ...

    • Computational study of SERT using new X-ray crystal structures and initial experimental verification of potential GABAB receptor compounds 

      Godtliebsen, Gustav (Master thesis; Mastergradsoppgave, 2018-05-15)
      Depression is currently one of the leading causes worldwide of suicide and disability, the most common treatment is antidepressants. Most antidepressants work by increasing the monoamine levels in the central nervous system by inhibition of the reuptake of monoamines into the presynaptic neuron, and thereby ensure accumulation of the monoamines in the synaptic cleft. The main target for most ...

    • Exploring mitochondrial quality control mechanisms and mitochondria-lipid droplet interactions in cardiac cell models 

      Godtliebsen, Gustav (Doctoral thesis; Doktorgradsavhandling, 2024-01-11)
      Mitochondria are the main energy producing units (organelles) in cardiac cells (cardiomyocytes). Cardiomyocytes have especially high mitochondrial content due to the heart’s continuous energy-intensive pumping. Studies of heart disease (such as heart failure) indicate that mitochondrial dysfunction is central to disease progression. There are many cellular mechanisms that protect mitochondria from ...

    • High-resolution visualization and assessment of basal and OXPHOS-induced mitophagy in H9c2 cardiomyoblasts 

      Godtliebsen, Gustav; Larsen, Kenneth Bowitz; Bhujabal, Zambarlal Babanrao; Opstad, Ida Sundvor; Nager Grifo, Mireia; Punnakkal, Abhinanda Ranjit; Kalstad, Trine; Olsen, Randi; Lund, trine; Prasad, Dilip K.; Agarwal, Krishna; Myrmel, Truls; Birgisdottir, Åsa birna (Journal article; Tidsskriftartikkel; Peer reviewed, 2023-07-05)
      Mitochondria are susceptible to damage resulting from their activity as energy providers. Damaged mitochondria can cause harm to the cell and thus mitochondria are subjected to elaborate quality-control mechanisms including elimination via lysosomal degradation in a process termed mitophagy. Basal mitophagy is a house-keeping mechanism fine-tuning the number of mitochondria according to the metabolic ...

    • Instance Segmentation of Microscopic Foraminifera 

      Johansen, Thomas Haugland; Sørensen, Steffen Aagaard; Møllersen, Kajsa; Godtliebsen, Gustav (Journal article; Tidsskriftartikkel; Peer reviewed, 2021-07-16)
      Foraminifera are single-celled marine organisms that construct shells that remain as fossils in the marine sediments. Classifying and counting these fossils are important in paleo-oceanographic and -climatological research. However, the identification and counting process has been performed manually since the 1800s and is laborious and time-consuming. In this work, we present a deep learning-based ...

    • Mitochondrial dynamics and quantification of mitochondria-derived vesicles in cardiomyoblasts using structured illumination microscopy 

      Opstad, Ida S.; Godtliebsen, Gustav; Ahluwalia, Balpreet Singh; Myrmel, Truls; Agarwal, Krishna; Birgisdottir, Åsa Birna (Journal article; Tidsskriftartikkel; Peer reviewed, 2021-11-12)
      Mitochondria are essential energy-providing organelles of particular importance in energy-demanding tissue such as the heart. The production of mitochondria-derived vesicles (MDVs) is a cellular mechanism by which cells ensure a healthy pool of mitochondria. These vesicles are small and fast-moving objects not easily captured by imaging. In this work, we have tested the ability of the optical ...

    • Physics-based machine learning for subcellular segmentation in living cells 

      Sekh, Arif Ahmed; Opstad, Ida Sundvor; Godtliebsen, Gustav; Birgisdottir, Åsa Birna; Ahluwalia, Balpreet Singh; Agarwal, Krishna; Prasad, Dilip K. (Journal article; Tidsskriftartikkel; Peer reviewed, 2021-12-15)
      Segmenting subcellular structures in living cells from fluorescence microscope images is a ground truth (GT)-deficient problem. The microscopes’ three-dimensional blurring function, finite optical resolution due to light diffraction, finite pixel resolution and the complex morphological manifestations of the structures all contribute to GT-hardness. Unsupervised segmentation approaches are quite ...

    • Three-dimensional structured illumination microscopy data of mitochondria and lysosomes in cardiomyoblasts under normal and galactose-adapted conditions 

      Opstad, Ida Sundvor; Godtliebsen, Gustav; Ströhl, Florian; Myrmel, Truls; Ahluwalia, Balpreet Singh; Agarwal, Krishna; Birgisdottir, Åsa birna (Journal article; Tidsskriftartikkel; Peer reviewed, 2022-03-23)
      This three-dimensional structured illumination microscopy (3DSIM) dataset was generated to highlight the suitability of 3DSIM to investigate mitochondria-derived vesicles (MDVs) in H9c2 cardiomyoblasts in living or fxed cells. MDVs act as a mitochondria quality control mechanism. The cells were stably expressing the tandem-tag eGFP-mCherry-OMP25-TM (outer mitochondrial membrane) which can be used ...